Molecular characterization of Pseudomonas aeruginosa isolates from Sudanese patients: A cross-sectional study

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is a gram-negative bacterium that is found widely in the environment and engages in various forms of interactions with eukaryotic host organisms. It is an opportunistic pathogen that is widely spread in humans, giving rise to a broad spectrum of infections in community and healthcare facilities. Sequencing of 16S rRNA worldwide has provided interesting and useful informationn Objectives Course Objective 16S rRNA gene sequence analysis is a robust tool for characterization of new pathogens in clinical specimens with suspected bacterial disease. The aim of this study was to characterize. Forty bacterial isolates were obtained from different clinical specimens (wound, urine and sputum) using enrichment selective media and biochemical tests to characterize and identify the bacteria as. Sequence analysis by BLASTn displayed high similarity and identity with. Our observation of isolates from different origin sites, further show the utility of 16s rRNA PCR amplification. This reveals the high specify of the primers and accuracy of the PCR. Thus, 16S rRNA sequencing can be used to identify genetically atypical n