Andrographolide, isolated from Andrographis paniculata, induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

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Many plant-derived products possess a potential for use in chemotherapy. For example, vincristine (VCR) and vinblastine—two natural alkaloids isolated from. Andro has been shown to have anti-tumor activities against solid and hematopoietic tumor cell lines, established from colon-, gastric-, liver-, breast-, and prostatic cancers, leukemia, and lymphoma (. THP-1 (RRID:CVCL_0006) is a permanent human monocytic cell line derived from an acute monocytic leukemia patient (. H929 (aka “NCI-H929”) (RRID:CVCL_1600) is a permanent human IgA-kappa-producing multiple myeloma cell line (. In the present study, we investigated the viability-reducing effects and their mechanisms of Andro on both THP-1 and H929 cells. The effects of Andro were compared with those of the common anti-cancer drugs VCR and Ara-C (aka cytarabine). VCR and Ara-C are widely used as chemotherapeutic agents against soft tissue tumors and hematopoietic tumors including acute leukemia, lymphoma, and multiple myeloma (n ... Objectives Course Objective : Andrographolide (Andro) is a diterpenoid component of the plant. : We studied the effects of Andro on the viability of the human leukemia monocytic cell line THP-1 and the human multiple myeloma cell line H929. Andro was compared with cytosine arabinoside (Ara-C) and vincristine (VCR), which are well-established therapeutics against hematopoietic tumors.. : Andro reduced the viability of THP-1 and H929 in a dose-dependent manner. H929 viability was highly susceptible to Andro, although only slightly susceptible to Ara-C. The agents Andro, Ara-C, and VCR each induced apoptosis, as shown by cellular shrinkage, DNA fragmentation, and increases in annexin V-binding, caspase-3/7 activity, reactive oxygen species (ROS) production, and mitochondrial membrane depolarization.